Haematology

Preferred Sample Type

Bethesda / Inhibitor Assay (Human, Porcine and Bovine Chromogenic)

Bethesda / Inhibitor Assay (Human, Porcine and Bovine Chromogenic)

Specimen Volume

2 x 3.5 mL (Vacuette tubes) or 2.7mL (BD Vacutainer tubes)

Specimen Transport

Standard transport to laboratory - State for the attention of Special Coagulation on request form

Sample Preparation

Samples must be filled to the black fill line (Vacuette tubes) or the frosted line (BD Vacutainer tubes) indicated on the sample tube. Any sample filled above or below this level will be rejected and a repeat sample will be required

Turnaround Time

21 days

Sample Processing In Laboratory

Sample should be centrifuged

Sample Stability

12 hours from venepuncture (ideally received no later than 4 hours after collection)

General Information

Note: For external requests please click Here for Coagulation Disorders request form. Please complete all information fully 

Samples that meet the following criteria will not be processed:

  • Haemolysed, clotted, underfilled or overfilled samples.
  • Samples >12 hours old from the time of collection.
  • Samples received from other hospital laboratories that have been frozen and thawed.
  • Samples which fail the minimum data set.

Assay Limitations:

  • If a patient has been treated with blood products e.g. factor concentrate, FEIBA, etc before performing coagulation inhibitor assays this should be taken into consideration when interpreting the data obtained.
  • It may also be useful to perform a lupus inhibitor screen when dealing with a suspected acquired coagulation factor inhibitor.

Patients on Emicizumab:

  • Inhibitor levels for patients treated with Emicizumab are processed using the Bovine Chromogenic Bethesda assay. Please clearly indicate on the request form if the patient is on Emicizumab. Note all positive samples are currently sent to Royal Hallamshire Hospital, Sheffield for confirmation.

 

Reference Range

<0.6 BU/mL

Specifications

  • EQA Status: ECAT
  • EQAS Scheme: Yes

Creation Date

Saturday, 24 November 2018

Modification Date

Thursday, 20 March 2025

General Information

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Copyright UHB Pathology 2025

Protection of Personal Information – Pathology Laboratories Services comply with the Trust Data Protection Policy and have procedures in place to allow the Directorate and it’s employees to comply with the Data Protection Act 1998 and associated best practice and guidance.

The laboratories at Queen Elizabeth Hospital, Heartlands Hospital, Good Hope Hospital and Solihull Hospital form part of the services provided by University Hospitals Birmingham and are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2022 standard. For a list of accredited tests and other information please visit the UKAS website using the following link: https://www.ukas.com/find-an-organisation/ 

  • Molecular Pathology is a UKAS accredited medical laboratory No. 8759
  • Biochemistry is a UKAS accredited medical laboratory No. 8910
  • Haematology and Transfusion is a UKAS accredited medical laboratory No. 8784
  • Clinical Microbiology is a UKAS accredited medical laboratory No. 8760
  • Cellular Pathology is a UKAS accredited medical laboratory No. 10141
  • United Kingdom Health Security Agency laboratory is a UKAS accredited medical laboratory No.8213

Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further test information, please visit the test database: https://qehbpathology.uk/test-database 

For further information contact Louise Fallon, Quality Manager, 0121 371 5962/ 0121 424 1235