Preferred Sample Type

Sterile Fluid

Suitable Specimen Types

Aspirate

Sterile Fluid

Specimen Volume

>2mL

Specimen Transport

Sample must be sent with a porter to the laboratory as soon as possible

Sample Preparation

Samples that are heavily blood stained or clotted will not be counted. Samples received in an EDTA tube only cannot be Gram stained or cultured.

Turnaround Time

24 hours (microscopy), 2 to 4 Days (Negative Report) 3 to 5 Days (Positive Report)

Sample Processing In Laboratory

All samples will have a Gram film. Samples are cultured for microorganisms.

Sample Stability

If transport is delayed, samples must be stored at ambient room temperature for a maximum of 8 hours.

General Information

  • The laboratory will examine samples of ascites, peritoneal dialysis fluid and synovial / bursa fluids. If urgent processing is required notify the laboratory using extension number 16520 during normal working hours or via switchboard at other times.
  • Samples must be received in a sterile universal container. If TB, viral or parasitic investigations are required, this should be clearly stated on the request form.
  • A cell count is performed on ascites and peritoneal dialysis fluids. To avoid samples clotting in transit, an additional EDTA tube may be sent with the universal container.
  • Synovial/bursa fluids are examined for the presence of birefringent crystals.
  • Sterile fluids may also be sent in blood culture bottles. It is not possible to perform cell counts or direct Gram’s stain on these samples. Please send an additional universal container and/or EDTA tube if these analyses are required. (See Blood Cultures for information on how to collect)

 

Information Sharing: If your sample needs to be referred or a notifiable disease is detected from your sample, your details and sample may be shared with the UK Health Security Agency (UKHSA). This is a legal requirement. The UKHSA is sponsored by the Department of Health and Social Care.

 

 

Specifications

  • EQA Status: Enrolled to UK NEQAS scheme and RCPAQAP (for synovial fluid crystals)
  • EQAS Scheme: Yes

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