Preferred Sample Type
Suitable Specimen Types
Special Collection Procedures ApplyAlkaline Phosphatase (ALP) Isoenzymes (APISO)
Specimen Volume
1.0mL bloodSpecimen Transport
Not applicableSample Preparation
Turnaround Time
4 weeksSample Processing In Laboratory
Centrifuge and separate serum. Perform total ALP analysis and GGT analysis beforehand and store sample at -20°C.Sample Stability
Store at -20°CGeneral Information
Alkaline Phosphatases (ALP) form a group of non-specific enzymes that hydrolyse a wide variety of ester orthophosphates under alkaline conditions. The total alkaline phosphatase activity represents several molecular forms or isoenzymes derived from a variety of human tissue sources. The biliary canaliculi of the liver, osteoblasts of bone, intestinal mucosa and the placenta are sources of alkaline phosphatase isoenzymes. Additional isoenzymes are associated with specific benign and malignant disorders.
An elevated total serum alkaline phosphatase is often one of the first biochemical abnormalities to be encountered in clinical investigation. Assessment of the isoenzyme fractions contributing to the elevated value gives valuable information in a variety of clinical conditions, such as hepatobiliary disease and bone disease.
Patient Preparation
No specific patient preparation is required for this test. The test can only be performed in serum (yellow or red top).
Reference Range
A qualitative report is provided. Reference range is not applicable.
Source of Reference Range
Not applicableSpecifications
- EQA Status: No EQA scheme available.
- EQAS Scheme: No