ALK IHC & FISH
Specimen Transport
Blocks individually wrapped and slides in protective container. Must be sent via recorded delivery.Sample Preparation
Slides dried at 37°C
Turnaround Time
IHC - 5-7 days & FISH - 7-10 daysGeneral Information
Anaplastic lymphoma kinase (ALK) gene fusions can be targeted with specific inhibitors, and translocation analysis using immunohistochemistry or Fluorescence In-Situ Hybridisation (FISH) is now standard alongside EGFR mutation analysis to determine 1st line treatment for lung NSCLC. In addition to ALK analysis.
- Paraffin block from biopsy, resection or cell block
- 3μm unstained mounted sections on charged slides for IHC
- 2μm unstained mounted sections on charged slides for FISH
Notes
Please note tissue handling, tissue fixation, time between cutting and staining and excessive drying can lead to loss of expression. Fixatives containing alcohol should not be used and use of Bouin’s fixative will preclude testing by FISH methods.
A copy of the original Pathology report where possible.
Specifications
- EQA Status: NEQAS