Haematology

Preferred Sample Type

ADAMTS13 Activity / Inhibitor (IgG)

ADAMTS13 Activity / Inhibitor (IgG)

Specimen Volume

3 x 3.5 mL (Vacuette tubes) or 2.7mL (BD Vacutainer tubes)

Specimen Transport

Standard transport to laboratory - State for the attention of Special Coagulation on request form

Sample Preparation

Samples must be filled to the black fill line (Vacuette tubes) or the frosted line (BD Vacutainer tubes) indicated on the sample tube. Any sample filled above or below this level will be rejected and a repeat sample will be required

Turnaround Time

ADAMTS13 Activity: 6 hours for urgent requests, 7 days for routine requests, ADAMTS13 Inhibitor: 21 days.

Sample Processing In Laboratory

Sample should be centrifuged

Sample Stability

Up to 72 hours from venepuncture

General Information

Note: If you suspect MAHA/TTP, please contact the UHB Haematology Consultant via Switchboard for urgent advice. Tel: 0121 627 200.

For urgent samples, Tel: 0121 371 5988 (Special Coagulation Lab at QE) or 0121 371 6920 out of hours. Samples can sent for processing 24 hours a day.

For external requests please click Here for ADAMTS13 request form. Please complete all information fully

Samples that meet the following criteria will not be processed:

  • Haemolysed, clotted, underfilled or overfilled samples.
  • Samples >12 hours old from the time of collection.
  • Samples received from other hospital laboratories that have been frozen and thawed.
  • Samples which fail the minimum data set.

Assay limitations:

  • Samples containing EDTA cannot be used because EDTA is a strong inhibitor of ADAMTS13 function

Underfilled samples for ADAMTS13 Activity only:

  • Underfilled samples CAN be accepted for ADAMTS13 Activity only providing the sample tube is at least half full.

Reference Range

Reference Ranges are dependent upon methodology:

ADAMTS13 Activity:

ACUSTAR (primary method): 62-118 IU/dL

ELISA (secondary method, used for confirmatory check of equivocal results): 38-110%

ADAMTS13 inhibitor (IgG):

Neg <12 U/mL

Borderline 12-15 U/mL

Positive >15 U/mL

Specifications

  • EQA Status: ECAT / NEQAS
  • EQAS Scheme: Yes

Creation Date

Saturday, 24 November 2018

Modification Date

Thursday, 20 March 2025

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Protection of Personal Information – Pathology Laboratories Services comply with the Trust Data Protection Policy and have procedures in place to allow the Directorate and it’s employees to comply with the Data Protection Act 1998 and associated best practice and guidance.

The laboratories at Queen Elizabeth Hospital, Heartlands Hospital, Good Hope Hospital and Solihull Hospital form part of the services provided by University Hospitals Birmingham and are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2022 standard. For a list of accredited tests and other information please visit the UKAS website using the following link: https://www.ukas.com/find-an-organisation/ 

  • Molecular Pathology is a UKAS accredited medical laboratory No. 8759
  • Biochemistry is a UKAS accredited medical laboratory No. 8910
  • Haematology and Transfusion is a UKAS accredited medical laboratory No. 8784
  • Clinical Microbiology is a UKAS accredited medical laboratory No. 8760
  • Cellular Pathology is a UKAS accredited medical laboratory No. 10141
  • United Kingdom Health Security Agency laboratory is a UKAS accredited medical laboratory No.8213

Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further test information, please visit the test database: https://qehbpathology.uk/test-database 

For further information contact Louise Fallon, Quality Manager, 0121 371 5962/ 0121 424 1235