Biochemistry

Preferred Sample Type

Lipoprotein a (Lp(a)) Genotyping (LIPOAG)

Suitable Specimen Types

EDTA Whole Blood

Lipoprotein a (Lp(a)) Genotyping (LIPOAG)

Specimen Volume

5 mL Whole Blood

Specimen Transport

Usual

Sample Preparation

Store whole blood together with bar-codes at 4 oC in LIPOAG pot in CSU.

Do not centriuge or freeze

Turnaround Time

3 Days

Sample Stability

4 oC in research rack in CSU

General Information

Lipoprotein(a) (Lp(a)) is a plasma lipoprotein which consists of an apo(B) containing LDL-like particle covalently linked to a large glycoprotein, apo(a). Elevated plasma concentrations of Lp(a) are a risk factor for coronary heart disease, cerebrovascular disease, atherosclerosis, thrombosis and stroke. Apo(a) is encoded by the LPA gene with variation in the LPA gene accounting for around 90% of the variation in Lp(a) levels with size of the genetically determined Kringle 4 type 2 (KIV-2) repeat in the LPA gene affecting the size of the apo(a) protein.

Small numbers of KIV-2 repeats, and hence small isoforms of apo(a), are associated with an increased risk of CVD and atherosclerosis and can give erroneously low levels of Lp(a) when measured on some assays.

This assay will determine the number of KIV-2 repeats via qPCR and will also determine the presence of absence of two SNPs, rs10455872 and rs3798220, which are associated with an increased odds ratios for CAD.


For more information, please see the links to the publicaitons below.

 

 

Patient Preparation

No preperation required.

Test will not be performed without a completed patient consent form and permission from the relevent consultant.

Notes

Please only place samples for Lp(a) genotyping in the CSU pot if it is clearly indicated on the request form (e.g. LIPOA GENOTYPING). All samples for immunochemical measurement of Lp(a) DO NOT require Lp(a) genotyping.

Any questions please contact Dr Alex Lawson on 43235 or the duty biochemist on bleep 2506

Reference Range

Interpretative comments will be attached to report.

Specifications

  • EQA Status: No EQA scheme available.
  • EQAS Scheme: Yes

Link to Further Information

http://www.nejm.org/doi/full/10.1056/NEJMoa0902604 http://jama.jamanetwork.com/article.aspx?articleid=184063

Creation Date

Friday, 03 August 2012

Modification Date

Sunday, 18 February 2018

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Protection of Personal Information – Clinical Laboratory Services comply with the Trust Data Protection Policy and have procedures in place to allow the Directorate and it’s employees to comply with the Data Protection Act 1998 and associated best practice and guidance.

University Hospitals Birmingham medical laboratories at Queen Elizabeth Hospital, Heartlands Hospital, Good Hope Hospital and Solihull Hospital are UKAS (United Kingdom Accreditation Service) accredited to the ISO 15189:2012 standard. For a list of accredited tests and other information please visit the UKAS website using the following link: https://www.ukas.com/find-an-organisation/

  • Molecular Pathology is a UKAS accredited medical laboratory No. 8759
  • Biochemistry is a UKAS accredited medical laboratory No. 8910
  • Haematology and Transfusion is a UKAS accredited medical laboratory No. 8784
  • Clinical Microbiology is a UKAS accredited medical laboratory No. 8760
  • Cellular Pathology is a UKAS accredited medical laboratory No. 10141
  • Musculoskeletal laboratory is a UKAS accredited medical laboratory No. 9897
  • Heartlands, Good Hope and Solihull Hospital pathology laboratories are a UKAS accredited medical laboratory No.8217.

Tests not appearing on the UKAS Schedule of Accreditation currently remain outside of our scope of accreditation. However, these tests have been validated to the same high standard as accredited tests and are performed by the same trained and competent staff.

For further test information, please visit the test database: http://qehbpathology.uk/test-database

For further information contact Louise Fallon, Quality Manager, 0121 371 5962